AMFR Knockout cell line (THP-1)

Gene Editing Services

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Red Cotton CRISPR Gene Editing Designer

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Red Cotton Gene knockout Project

AMFR Knockout cell line (THP-1) Order

Catalog#

YKO-HT0258

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

AMFR Knockout cell line (THP-1)

Species

Gene Name

AMFR

Gene ID

267

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the AMFR gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, AMFR gene knockout AMFR Knockout cell line (THP-1) will be expanded and cryopreserved.